Advances in Exercise and Sports Physiology
Volume 7, Number 4, December 2001
The influence of a dietary amino acid mixture on blood fat metabolites during a one momth walking exercise.

T. Abe (RIKEN), M. Yano (Teikyo Univ.), H. Sasagawa (Hoshi Chem. Co.)

The change in blood fat metabolites was analyzed after daily dietary administration of a six amino acid mixture, based on the Vespa Amino Acid Mixture derived from Hornet Larval Saliva – D.A. (Dietary Amino Acid); proline: alanine: valine: isoleucine: leucine: lysine.

The experiment was carried out using the double blind method.

The 18 subjects were healthy male and female volunteers who were on average 23.7 years old and 60.5 kg.

Half of the subjects ingested 1.5g of D.A. twice per day on an empty stomach. The other half of the subjects ingested the same amount of placebo under the same conditions.

Both subjects consumed 1,400 Kcal and walked 6,400 steps a day.

Body weight and body fat were measured each day.

Before intake and after one month, blood was drawn 4 hours after breakfast. Subjects did not eat between breakfast and bloodwork.

Blood indicies related to fat metabolism; total lipid, total cholesterol, HDL cholesterol, phospholipids, B-lipoprotein, free cholesterol, cholesterol ratio, non-esterified fatty acids and peroxidized lipids were measured.

In the subjects administered placebo, indicies did not show significant differences before and after administration.

Subjects administered D.A. showed significant decreases (p<0.05) in the values of total lipids, phospholipids, HDL cholesterol and cholesterol ratio.

On the other hand, non-esterified fatty acids and peroxidized lipids were significantly increased (p<0.05).

Furthermore, low decreases of body weight and body fat were observed in the D.A. group during the period, but not the placebo group.

Results demonstrated the continuous intake of D.A. during one month increased fat oxidation and reduced the concentration of some blood lipid components.